Crosslinks and crosstalkHuman cancer syndromes and DNA repair defects
نویسندگان
چکیده
منابع مشابه
DNA Interstrand Crosslinks Repair in Mammalian Cells
We studied the formation of double strand breaks (DSBs) as intermediates in the repair of DNA interstrand crosslinks (ICLs) by homologous recombination (HR). The plasmid EGFP-N1 was crosslinked with trioxsalen to give one ICL per plasmid on average. HeLa cells were transfected with the crosslinked plasmids and the ICL repair was monitored by following the restoration of the GFP expression. It w...
متن کاملDNA repair pathways and hereditary cancer susceptibility syndromes.
Every living organism is exposed to numerous genomic insults on a daily basis as a consequence of cellular metabolism and exposure to environmental agents capable of interacting with the genome (e.g. chemicals, toxins, pollutants, UV and ionizing radiation) (1). Maintenance of the integrity of the genome is paramount to the survival and propagation of a species and involves the continuous activ...
متن کاملMismatch repair and nucleotide excision repair proteins cooperate in the recognition of DNA interstrand crosslinks
DNA interstrand crosslinks (ICLs) are among the most cytotoxic types of DNA damage, thus ICL-inducing agents such as psoralen, are clinically useful chemotherapeutics. Psoralen-modified triplex-forming oligonucleotides (TFOs) have been used to target ICLs to specific genomic sites to increase the selectivity of these agents. However, how TFO-directed psoralen ICLs (Tdp-ICLs) are recognized and ...
متن کاملNucleotide excision repair and homologous recombination systems commit differentially to the repair of DNA-protein crosslinks.
DNA-protein crosslinks (DPCs)-where proteins are covalently trapped on the DNA strand-block the progression of replication and transcription machineries and hence hamper the faithful transfer of genetic information. However, the repair mechanism of DPCs remains largely elusive. Here we have analyzed the roles of nucleotide excision repair (NER) and homologous recombination (HR) in the repair of...
متن کاملRepair of DNA-polypeptide crosslinks by human excision nuclease.
DNA-protein crosslinks are relatively common DNA lesions that form during the physiological processing of DNA by replication and recombination proteins, by side reactions of base excision repair enzymes, and by cellular exposure to bifunctional DNA-damaging agents such as platinum compounds. The mechanism by which pathological DNA-protein crosslinks are repaired in humans is not known. In this ...
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ژورنال
عنوان ژورنال: Cancer Cell
سال: 2004
ISSN: 1535-6108
DOI: 10.1016/s1535-6108(04)00338-1